Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73605634" target="_blank" >RIV/61989592:15110/20:73605634 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/20:00116484 RIV/00098892:_____/20:N0000122
Result on the web
<a href="https://www.mdpi.com/2075-4418/10/8/564" target="_blank" >https://www.mdpi.com/2075-4418/10/8/564</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/diagnostics10080564" target="_blank" >10.3390/diagnostics10080564</a>
Alternative languages
Result language
angličtina
Original language name
Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma
Original language description
Noninvasive fetal RHD genotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetal RHD genotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetal RHD genotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from the RHD gene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of the RHD genotyping. The RHD genotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict the RHD genotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetal RHD allele from the plasma of RhD-negative pregnant women.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/NT12225" target="_blank" >NT12225: Determining fetal RHD and KELL genotype from peripheral blood of pregnant women</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Diagnostics
ISSN
2075-4418
e-ISSN
—
Volume of the periodical
10
Issue of the periodical within the volume
8
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
"'564(1)'"-"'564(15)'"
UT code for WoS article
000568020600001
EID of the result in the Scopus database
2-s2.0-85090251997