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IDH1/2 mutations in patients with diffuse gliomas: A single centre retrospective massively parallel sequencing analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F22%3A73610228" target="_blank" >RIV/61989592:15110/22:73610228 - isvavai.cz</a>

  • Alternative codes found

    RIV/70883521:28150/21:63535058 RIV/00098892:_____/22:10157737

  • Result on the web

    <a href="https://journals.lww.com/appliedimmunohist/Fulltext/2022/03000/IDH1_2_Mutations_in_Patients_With_Diffuse_Gliomas_.4.aspx" target="_blank" >https://journals.lww.com/appliedimmunohist/Fulltext/2022/03000/IDH1_2_Mutations_in_Patients_With_Diffuse_Gliomas_.4.aspx</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1097/PAI.0000000000000997" target="_blank" >10.1097/PAI.0000000000000997</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    IDH1/2 mutations in patients with diffuse gliomas: A single centre retrospective massively parallel sequencing analysis

  • Original language description

    Patients below 55 years were genetically studied because the prevalence of isocitrate dehydrogenase 1 (IDH1) decreases in older patients and on grounds of cost-effectiveness, as suggested by the World Health Organization (WHO) in 2016. The aim of our study was to use novel massively parallel sequencing (MPS) approaches to examine rare variants of IDH1/2 in Czech diffuse astrocytic and oligodendroglial tumors (gliomas) patients below 55 years of age who had been immunohistochemically (IHC) diagnosed as IDH1 R132H negative. The IHC IDH1 status (wild type or mutant) of 275 tissue samples was analyzed using antibodies against the IDH1 R132H protein. Sixty-three samples of 55 years old patients with IHC IDH1 WT status were genotyped using two different MPS technologies to detect rare IDH1 and IDH2 variants. The tiered IHC (60 positive) and molecular (10 positive) approach thus revealed that 70 of the 275 samples (25%) bore IDH1/IDH2 mutations. The combined molecular and IHC approach thus revealed that 70 of the 275 samples (25%) considered in the study bore IDH1/IDH2 mutations. IHC detection of the IDH1 R132H variant should be routinely complemented with MPS to detect rare IDH1/2 variants in glioma patients below 55 years of age with negative IHC result of IDH R132H variant.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30109 - Pathology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    APPLIED IMMUNOHISTOCHEMISTRY &amp; MOLECULAR MORPHOLOGY

  • ISSN

    1541-2016

  • e-ISSN

    1533-4058

  • Volume of the periodical

    30

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    6

  • Pages from-to

    178-183

  • UT code for WoS article

    000766274300005

  • EID of the result in the Scopus database

    2-s2.0-85121040979