Generation of singlet oxygen by porphyrin and phthalocyanine derivatives regarding the oxygen level

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F22%3A73619208" target="_blank" >RIV/61989592:15110/22:73619208 - isvavai.cz</a>

Result on the web

<a href="https://jms.ump.edu.pl/index.php/JMS/article/view/752/978" target="_blank" >https://jms.ump.edu.pl/index.php/JMS/article/view/752/978</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20883/medical.e752" target="_blank" >10.20883/medical.e752</a>

Result language

angličtina

Original language name

Generation of singlet oxygen by porphyrin and phthalocyanine derivatives regarding the oxygen level

Original language description

Background. The principle of photodynamic effect is based on the combined action of photosensitiser, molecular oxygen and light, which produce various reactive oxygen species and are associated with significant cellular damage. Singlet oxygen is one of the most serious representatives, which is characterised by powerful oxidising properties. Moreover, concomitant hyperbaric oxygen treatment can support these effects. Therefore, the subject of our study was to compare the yields of singlet oxygen for four different photosensitizers in dependency on the oxygen concentration.Material and methods. Four different photosensitizers 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetra(p-toluenesulfonate), tetramethylthionine chloride, 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin zinc(II) and zinc phthalocyanine disulfonate were investigated to determine the yield of singlet oxygen in PBS by Singlet Oxygen Sensor Green reagent under different partial pressures of oxygen (0.4 and 36 mg/l).Results. There were no noticeable shifts in the excitation and emission fluorescence spectra regarding the oxygen concentration. Concerning the same molar concentration of photosensitizers the production of singlet oxygen was highest for 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin zinc(II), where the rate of the fluorescence change was more than 3 times higher than that obtained for 5,10,15,20-tetrakis(1-methyl-4-pyridinio) porphyrin tetra(p-toluenesulfonate). On the other hand, zinc phthalocyanine disulfonate showed the lowest yield in singlet oxygen production.Conclusions. Singlet oxygen production, within the range of oxygen concentrations achievable in tissues under normoxia or hyperoxia, does not depend on these concentrations. However, the singlet oxygen generation is signifi cantly influenced by the type of photosensitizer, with the highest yield belonging to 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrin zinc(II).

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

10303 - Particles and field physics

Project

<a href="/en/project/NU21-09-00357" target="_blank" >NU21-09-00357: Advantages of photodynamic and sonodynamic therapy in multidrug-resistant microorganisms</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Medical Science

ISSN

2353-9798

e-ISSN

2353-9801

Volume of the periodical

91

Issue of the periodical within the volume

4

Country of publishing house

PL - POLAND

Number of pages

9

Pages from-to

278-286

UT code for WoS article

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EID of the result in the Scopus database

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