Amine oxidase based biosensor for histamine detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F00%3A00001152" target="_blank" >RIV/61989592:15310/00:00001152 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Amine oxidase based biosensor for histamine detection
Original language description
This work reports on the development and optimization of amperometric biosensors based on the enyzme amine oxidase (AO) for the detection of histamine, a well-known biomarker for food freshness. Biosensor characteristics were evaluated in a flow injection (FI) analysis line, operated at +200 mV (vs. Ag/AgCl/0.1 M KCl). Two different biosensor designs were considered, one based on adsorbed AO on graphite electrodes, the detection being based on a direct electron transfer (DET) mechanism, whereas the second one based on a Os-bipyridine modified redox polymer using a mediated electron transfer (MET) pathway. Both electrode designs were able to detect histamine in mM range, however, the [osmium(4,4´-dimethylbipyridine)2Cl]+/2+ complexed with poly(1-vinylimidazole) (PVI13-dmeOs) based electrodes showed superior characteristics with regard to stability, selectivity and linear range. These electrodes were characterized by a detection limit of 2.2 mM (calculated as three times the signal-to-no
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/GA203%2F97%2F0097" target="_blank" >GA203/97/0097: Molecular,structural and physiological properties of plant amine and cytokinine oxidases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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