A molecular survey to identify phytoplasmas associated with apple trees showing different disease symptoms.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00001190" target="_blank" >RIV/61989592:15310/01:00001190 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
A molecular survey to identify phytoplasmas associated with apple trees showing different disease symptoms.
Original language description
Apple trees showing a diverse range of symptoms from the Czech Republic and the UK were tested several times during the year to monitor and idntify phytoplasmas, The trees were examinated by nested PCR using broad spectrum or group specific primers to amplify phytoplasma 16SrDNA, or 16SrDNA plus spacer region. The identity of PCR products was confirmed by RFLP analyses. Several cutivars from the Czech Republic showing symptoms of rubbery wood, apple proliferation, or leaf deformation and reddening wereexamined, together with plants of the cv Lord Lambourne from the UK showing rubbery wood symptoms. Of the plants tested only a few were positive in nested PCR. The results suggest that appe trees can harbour phytoplasmas from group 16SrI-B, 16SrI-C, 16 SrX-a and 16SrXII-A. It is also apparent that phytoplasmas belonging to 16SrX-A group, typically associated with apple proliferation symptoms, could also occur in symptomless apple trees or in trees with symptoms other than typical apple pr
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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