Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00001214" target="_blank" >RIV/61989592:15310/01:00001214 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Localization of male-specifically expressed MROS genes on Silene latifolia by PCR on flow-sorted sex chromosomes and autosomes.

Original language description

The dioecious white campion Silene latifolia (syn. Melandrium album) has heteromorphic sex chromosomes, XX in females and XY in males, that are larger than the autosomes and enable their separation by flow sorting. The group of MROS-genes, the first male-specifically expressed genes in dioecious plants, was recently identified in S. latifolia. To localize the MROSgenes, we used the flow-sorted X chromosomes and autosomes as a template for PCR with internal primers. Our results indicate that the MROS_Y gene is located in at least two copies tandemly arranged on the X chromosome with additional copy(ies) on the autosome(s), while MROS1, MROS2, and MROS4 are exclusively autosomal. The specificity of PCR products was checked by digestion with a restrictionenzyme or reamplification using nested primers. Homology search of databases has shown the presence of five MROS3 homologues in A. thaliana, four of them arranged in two tandems, each consisting of two copies. We conclude that MROS3 is a

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/GV521%2F96%2FK117" target="_blank" >GV521/96/K117: Novel approaches for effective analysis and mapping of genomes in cultivated plants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genetics resources and crop evolution

ISSN

0925-9864

e-ISSN

—

Volume of the periodical

N

Issue of the periodical within the volume

158

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

1269-1277

UT code for WoS article

—

EID of the result in the Scopus database

—