Cytokinin oxidase/dehydrogenase genes in barley and wheat - Cloning and heterologous expression
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002111" target="_blank" >RIV/61989592:15310/04:00002111 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/04:00109747
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cytokinin oxidase/dehydrogenase genes in barley and wheat - Cloning and heterologous expression
Original language description
The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described in this work. Transformation of both genes into Arabidopsis and tobacco showed that at least one of the genes codes for a functional enzyme, as its expression caused a cytokinin-deficient phenotype in the heterologous host plants. Additional cloning of two gene fragments, and an in silico search in the public expressed sequence tag clone databases, revealed the presence of at least 13 more members ofthe CKX gene family in barley and wheat. The expression of three selected barley genes was analyzed by RT-PCR and found to be organ-specific with peak expression in mature kernels. One barley CKX (HvCKX2) was characterized in detail after heterologous expression in tobacco. Interestingly, this enzyme shows a pH optimum at 4.5 and a preference for cytokinin ribosides as substrates, which may indicate its vacuolar targeting. Different substrate specificities, and the pH profiles of cytokin
Czech name
Cytokininoxidasa/dehydrogenasa v ječmeni a pšenici - klonování a heterologní exprese
Czech description
Práce je věnována klonování a heterologní expresi cytokininoxidasy/dehydrogenasy v ječmeni a pšenici.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Biochemistry
ISSN
0014-2956
e-ISSN
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Volume of the periodical
271
Issue of the periodical within the volume
20
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
3990-4002
UT code for WoS article
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EID of the result in the Scopus database
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