New HPLC/MS approach to analyses of phenolics in plants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002117" target="_blank" >RIV/61989592:15310/04:00002117 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
New HPLC/MS approach to analyses of phenolics in plants
Original language description
The separation, identification and quantitative analysis of phenolic acids are necessary because of their importance as non-steroid antiphlogistic drugs, semi-products of biosynthesis of aromatic amino-acids in plants and natural products with antioxidative and anticarcinogenic abilities. A method was developed for LC/MS analysis of eighteen phenolic acids in plant material after methanolic extraction. Different stacionary (C8, C18, Phenyl-Hexyl) and many mobile phases were tested during an optimisationprocess. Two fundamentally different atmospheric pressure ionization interfaces (chemical and electrospray ionizations) were also compared in terms of ionization effectivity and limits of detection. The most suitable method for analysis of phenolic acids was carried out after combining Fenyl-Hexyl column, gradient elution (acetonitrile - 5 mmol.L-1 HCOOH) and negative mode electrospray ionization. A linear dynamic range of 1 - 300 pmol injected was found for the optimised method, with d
Czech name
Nová HPLC/MS metoda pro stanovení fenolických látek v rostlinnách
Czech description
Je uvedena nová HPLC/MS metoda pro stanovení fenolických látek v rostlinnách.
Classification
Type
D - Article in proceedings
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
4th International symposium on chromatography of natural products
ISBN
83-916900-1-6
ISSN
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e-ISSN
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Number of pages
236
Pages from-to
165
Publisher name
Skubiszewski Medical University of Lublin
Place of publication
Lublin-Kazimerz Dolny
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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