Analytical derivatization - a tool for determination of orotic acid

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002132" target="_blank" >RIV/61989592:15310/04:00002132 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/04:00000802 RIV/61989592:15110/04:00001902

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Analytical derivatization - a tool for determination of orotic acid

Original language description

Derivatization of orotic acid (OA) into various forms (ttimethylsilylderivate, alkyl ester and per-methylated derivate) and their evaluation by GUMS is described. The tested approach includes ion-exchange SPE clean-up, evaporation and chemical reaction with different types of derivatization agents (N,O-bis-(trimethylsilyl)trifluoroacetamide with trimethylehlorosilane, butanol with acetylchloride and ethereal solution of diazomethane). Derivate originated in the reaction with diazomethane was used for determination of urinary orotic acid by GC/MS. Detection limit of 0.28 mumol l(-1) was reached using the ion 82 m/z in single ion monitoring (SIM) mode. Linearity of the method was tested within the range of 3.4-2503.4 mumol l(-1) covering physiological and pathological levels of orotic acid in urine sample. Recoveries were within the range 93.7-110.6%. Application of the method on the patient with defect of ornithine transcarbamylase (OTC) was demonstrated as well. (C) 2003 Elsevier B.V.

Czech name

Analytická derivatizace - nástroj pro stanovení kyseliny orotové

Czech description

Bylo studováno využití analytická derivatizace jako nástroje pro stanovení kyseliny orotové.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GP303%2F02%2FD010" target="_blank" >GP303/02/D010: Application of capillary electrophoresis for analysis of biological materials</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

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Volume of the periodical

799

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

303-309

UT code for WoS article

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EID of the result in the Scopus database

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