Application of Q-TOF technology in plant hormone research

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002217" target="_blank" >RIV/61989592:15310/04:00002217 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Application of Q-TOF technology in plant hormone research
Original language description
Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Novák et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and col
Czech name
Použití Q-TOF technologie při studiu rostlinných hormonů
Czech description
Kombinace HPLC nebo kapilární kapalinové chromatografie (CapLC) s Q-TOF dává nové možnosti ve výzkumu rostlinných hormonů.

Project
<a href="/en/project/GA522%2F03%2F0323" target="_blank" >GA522/03/0323: Investigation of algae and cyanobacteria as producers of biologically active substances</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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