The gradient of exciting radiation within a sample affects the relative height of steps in the fast chlorophyll a fluorescence rise

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002282" target="_blank" >RIV/61989592:15310/04:00002282 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The gradient of exciting radiation within a sample affects the relative height of steps in the fast chlorophyll a fluorescence rise

Original language description

Measurement of the chlorophyll (Chl) a fluorescence rise (FR) under higher exciting irradiance (El), the O-J-I-P transient, or under lower irradiance, the O-I-P transient, is a routinely used method to access photosystem 2 function in thylakoid membranesof chloroplasts. Our measurements with a suspension of pea thylakoid membranes showed that the relative heights of the J and I steps in the FR depended not only on El but also on the concentration and thickness of the sample. We explain this effect as aconsequence of the gradient of El within the sample. We tested this suggestion by theoretical simulations of the FR based on the model that was previously used for simulation of the FR considering in addition the gradient of El within the sample. Our theoretical results correspond well with the experiments. The irradiance gradient effect may influence measured FR significantly and this fact should be taken into consideration in the interpretation of measured FRs.

Czech name

Gradient excitovaného záření bez ovlivnění vzorku relativní výškou kroku rychlosti vzrůstu fluorescence ve chlorofylu

Czech description

Byl studován gradient excitovaného záření bez ovlivnění vzorku relativní výškou kroku rychlosti vzrůstu fluorescence ve chlorofylu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GP204%2F02%2FP071" target="_blank" >GP204/02/P071: Changes in statistical distributions of chlorophyll fluorescence parameters caused by a stress of photosynthetic apparatus of plants</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Photosynthetica

ISSN

0300-3604

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

12

Pages from-to

161-172

UT code for WoS article

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EID of the result in the Scopus database

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