Cytokinin N-glucosylation inhibitors suppress deactivation of exogenous cytokinins in radish, but their effect on active endogenous cytokinins is counteracted by other regulatory mechanisms

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002395" target="_blank" >RIV/61989592:15310/04:00002395 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/04:00104355

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cytokinin N-glucosylation inhibitors suppress deactivation of exogenous cytokinins in radish, but their effect on active endogenous cytokinins is counteracted by other regulatory mechanisms

Original language description

The prolongation of the effect of exogenous cytokinins by inhibition of their inactivation was studied in Raphanus sativus L. cv. Rampouch. As in radish the main way of cytokinin inactivation is their N-glucosylation, inhibitors of this pathway, papaverine, theophylline and olomoucine, as well as two olomoucine analogues, bohemine and roscovitine, were tested. The latter ones, which function as potent inhibitors of cyclin-dependent kinases, have also been found to effectively inhibit cytokinin N-glucosylation. Incubation (24 h) of de-rooted radish seedlings with inhibitors resulted in c. 50% decrease of the conversion of model cytokinins, [(3)H]dihydrozeatin and [(3)H]N(6)-benzyladenine, to the corresponding 7N-glucosides. Simultaneously the level of the non-metabolized cytokinin bases was elevated. The activity of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) was suppressed in a dose dependent manner. The concentration of physiologically active endogenous cytokinins was not increased

Czech name

Inhibitory N-glukosylace cytokininů snižují deaktivaci exogenních cytokininů u ředkve, ale jejich efekt na aktivní endogenní cytokininy je ovlivňován jinými regulačními machanismy

Czech description

Inhibitory N-glukosylace cytokininů snižují deaktivaci exogenních cytokininů u ředkve, ale jejich efekt na aktivní endogenní cytokininy je ovlivňován jinými regulačními machanismy.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiologia Plantarum

ISSN

0031-9317

e-ISSN

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Volume of the periodical

121

Issue of the periodical within the volume

2

Country of publishing house

DK - DENMARK

Number of pages

8

Pages from-to

215-222

UT code for WoS article

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EID of the result in the Scopus database

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