The use of mutagens to increase the efficiency of the androgenic progeny production in Solanum nigrum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F05%3A00001832" target="_blank" >RIV/61989592:15310/05:00001832 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/05:00024533
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The use of mutagens to increase the efficiency of the androgenic progeny production in Solanum nigrum
Original language description
Pollen embryogenesis was successfully induced in Solanum nigrum L. (2n=6x=72). Stimulation of androgenesis expressed as the frequency of androgenic responsive anthers was observed after 10 and 20 mM ethyl methanesulphonate (EMS), 10 and 20 mM sodium azide (NaN3) and 0.2 mM N-nitroso-N-methylurea (MNU) treatment applied on seeds for 24 h. The frequency of androgenesis on the medium with sucrose was higher than on the medium with maltose. Androgenic regenerants originated also in the anthers collected from donor plants where survival after mutagenic treatment was lower than 50 %. Green haploid (3x), aneuploid (to 8x) and dihaploid (6x) plants were obtained. The high frequency of aneuploids among androgenic plants is explained by cell division irregularities in microsporial calli.
Czech name
Využití mutagenů ke zvýšení produkce androgenního potomstva u Solanum nigrum
Czech description
Pylová embryogeneze byla úspěšně indukována u Solanum nigrum L. (2n=6x=72). Stimulace androgeneze vyjádřená jako frekvence androgenně responzivních prašníků byla pozorována po 24h aplikaci 10 a 20 mM ethyl methanesulphonatu (EMS), 10 a 20 mM sodium azide(NaN3) a 0.2 mM N-nitroso-N-methylurey (MNU). Frekvence androgeneze na médiu se sacharozou byla vyšší než na médiu s maltozou. Zelení haploidi (3x), aneuploidi (až 8x) a dihaploidi (6x) byli získáni. vysoká frekvence aneuploidů mezi androgenními rostlinami může být vysvětlena nepravidelnostmi v buněčném cyklui na úrovni mikrosporiálního kalusu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA521%2F01%2F1383" target="_blank" >GA521/01/1383: Using of haploidization and polyploidization in interspecific hybridization of wild and culture potato species for obtaining of resistance resources a</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biologia Plantarum
ISSN
0006-3134
e-ISSN
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Volume of the periodical
49
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
181-186
UT code for WoS article
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EID of the result in the Scopus database
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