Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003084" target="_blank" >RIV/61989592:15310/06:00003084 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/06:00081794

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

Original language description

Musa spp ( bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. This study determined the ploidy levels and genome composition of the Musa germplasm collection, constituting over 300 accessions, at the International Institute of Tropical Agriculture in Nigeria and Uganda. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels, while genome composition was ascertained with RAPD markers that are specific for the A and B genomes of Musa. It was determined that at least 8% of the plants in thegermplasm collection were miss-classified in terms of ploidy and/or genome composition. The cultivars 'Pisang awak', 'Foulah 4' and 'Nzizi', previously classified as triploids, were found to be tetraploids by flow cytometry

Czech name

Ploidy and genome composition of Musa germplasm at the International Institute of Tropical Agriculture (IITA)

Czech description

Musa spp ( bananas and plantains) constitute a hybrid-polyploid complex and are classified according to different genome compositions such as AA, BB, AB, AAA, AAB, ABB, AAAA, ABBB, AAAB and AABB. Knowledge of ploidy and exact genome compositions of the parental material is essential for Musa breeding. This study determined the ploidy levels and genome composition of the Musa germplasm collection, constituting over 300 accessions, at the International Institute of Tropical Agriculture in Nigeria and Uganda. Flow cytometric analysis of nuclear DNA content was used to estimate ploidy levels, while genome composition was ascertained with RAPD markers that are specific for the A and B genomes of Musa. It was determined that at least 8% of the plants in thegermplasm collection were miss-classified in terms of ploidy and/or genome composition. The cultivars 'Pisang awak', 'Foulah 4' and 'Nzizi', previously classified as triploids, were found to be tetraploids by flow cytometry

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LC06004" target="_blank" >LC06004: Integration of research activities to study the plant genome</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

African Journal of Biotechnology

ISSN

1684-5315

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

13

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

1224-1232

UT code for WoS article

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EID of the result in the Scopus database

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