Evidence that cytochrome b559 is involved in superoxide production in Photosystem II: effect of synthetic short-chain plastoquinones in a cytochrome b559 tobacco mutant.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003238" target="_blank" >RIV/61989592:15310/06:00003238 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Evidence that cytochrome b559 is involved in superoxide production in Photosystem II: effect of synthetic short-chain plastoquinones in a cytochrome b559 tobacco mutant.

Original language description

Light-induced production of superoxide (O2.-) in spinach PSII membrane particles was studied using EPR spin-trapping spectroscopy. The presence of exogenous plastoquinones with a different side chain length enhanced O2.- production in the following order: PQ-1>PQ-2>>PQ-9. In PSII membrane particles isolated from tobacco mutant of cytochrome b559 (cyt b559) carrying single-point mutation in the b-subunit, which has also reduced amount of the a-subunit, the effect of PQ-1 was smaller than in the wild type. The increase in LP cyt b559 content, induced by incubation of spinach PSII membrane particles at low pH, resulted in a significant increase in O2.- formation in the presence of PQ-1, whereas it had little effect on O2.- production in the absence of PQ-1. The enhancement of O2.- formation induced by PQ-1 was not abolished by DCMU. Under anaerobic conditions, the dark oxidation of LP cyt b559 increased as the pH was decreased. The presence of molecular oxygen significantly enhanced dark

Czech name

Důkaz podílu cytochromu b559 na produkci superoxidu ve fotosystému II: efekt syntetických krátkých plastochinonů na tabáku s mutovaným cytochromem b559.

Czech description

Light-induced production of superoxide (O2.-) in spinach PSII membrane particles was studied using EPR spin-trapping spectroscopy. The presence of exogenous plastoquinones with a different side chain length enhanced O2.- production in the following order: PQ-1>PQ-2>>PQ-9. In PSII membrane particles isolated from tobacco mutant of cytochrome b559 (cyt b559) carrying single-point mutation in the b-subunit, which has also reduced amount of the a-subunit, the effect of PQ-1 was smaller than in the wild type. The increase in LP cyt b559 content, induced by incubation of spinach PSII membrane particles at low pH, resulted in a significant increase in O2.- formation in the presence of PQ-1, whereas it had little effect on O2.- production in the absence of PQ-1. The enhancement of O2.- formation induced by PQ-1 was not abolished by DCMU. Under anaerobic conditions, the dark oxidation of LP cyt b559 increased as the pH was decreased. The presence of molecular oxygen significantly enhanced dark

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

—

Project

<a href="/en/project/1K04103" target="_blank" >1K04103: Reactive oxygen species in photosynthesis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical Journal

ISSN

0264-6021

e-ISSN

—

Volume of the periodical

397

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

321-327

UT code for WoS article

—

EID of the result in the Scopus database

—