The application of Miniplex primer sets in the analysis of degraded DNA from human skeletal remains
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A73537340" target="_blank" >RIV/61989592:15310/06:73537340 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1556-4029.2006.00077.x" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1556-4029.2006.00077.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1556-4029.2006.00077.x" target="_blank" >10.1111/j.1556-4029.2006.00077.x</a>
Alternative languages
Result language
angličtina
Original language name
The application of Miniplex primer sets in the analysis of degraded DNA from human skeletal remains
Original language description
New set of multiplexed PCR primers has been applied to the analysis of human skeletal remains to determine their efficacy in analyzing degraded DNA. These primer sets, known as Miniplexes, produce shorter amplicons (50-280 base pairs (bp)) than standard short tandem repeat (STR) kits, but still utilize the 13 CODIS STR loci, providing results that are searchable on national DNA databases. In this study, a set of 31 different human remains were exposed to a variety of environmental conditions, extracted, and amplified with commercial and Miniplex DNA typing kits. The amplification efficiency of the Miniplex sets was then compared with the Promega PowerPlex(T) 16 system. Sixty-four percent of the samples generated full profiles when amplified with the Miniplexes, while only 16% of the samples generated full profiles with the Powerplex((R)) 16 kit. Complete profiles were obtained for 11 of the 12 Miniplex loci with amplicon sizes less than 200 bp. These data suggest smaller PCR amplicons may provide a useful alternative to mitochondrial DNA for anthropological and forensic analysis of degraded DNA from human skeletal remains.
Czech name
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Czech description
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Classification
Type
J<sub>ost</sub> - Miscellaneous article in a specialist periodical
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Forensic Sciences
ISSN
0022-1198
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
351-356
UT code for WoS article
000235841900018
EID of the result in the Scopus database
2-s2.0-33644925270