Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004841" target="_blank" >RIV/61989592:15310/07:00004841 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Hydroxyl radicals are not the protagonists of UV-B induced damage in isolated thylakoid membranes.
Original language description
The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (-OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation ofterephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm/-2), -OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of -OH was still observed in thylakoids, even though no free hydrogen peroxide was detected.
Czech name
Hydroxylové radikály nejsou protagonisté poškození izolovaných thylakoidních membrán pomocí UV-B.
Czech description
V práci bylo zjištěno, že v ranném stádiu působeni UV-B produkce reaktivních forem kyslíku (hydroxalové radikály a peroxid vodíku) závisí na elektronovém transportu a ne výhradně na UV-B.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Functional Plant Biology
ISSN
1445-4408
e-ISSN
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Volume of the periodical
34
Issue of the periodical within the volume
12
Country of publishing house
AU - AUSTRALIA
Number of pages
10
Pages from-to
1112-1121
UT code for WoS article
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EID of the result in the Scopus database
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