Methods of isolation of Cucumis sativus and C. melo pollen grains and their utilization in in vitro pollination

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005259" target="_blank" >RIV/61989592:15310/08:00005259 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Methods of isolation of Cucumis sativus and C. melo pollen grains and their utilization in in vitro pollination

Original language description

Optimizing manipulation with pollen grains is necessary for successful in vitro pollination. This pollination can be used for overcoming crossing barriers in interspecific hybridization in the genus Cucumis. Pollen grains from Cucumis sativus and C. melowere isolated by various methods. Different techniques of centrifugation were compared with direct isolation of pollen grains from anthers. The viability of pollen grains was evaluated and their ability to form pollen tubes was tested. Three isolation media (NLN, YST and VB) and two cultivation media (YS and CP) were used. Then pollen grains were cultivated with ovules of C. sativus and the level of regeneration was observed. The growing and green ovules were considered as the lowest level and the highest level was the formation of calli.

Czech name

Metody izolace pylových zrn Cucumis sativus a C. melo a jejich využití v in vitro opylování

Czech description

Optimalizace manipulace s pylovými zrny je důležitá pro řadu biotechnologických metod včetně in vitro opylování. To může být využito pro překonání hybridizačních bariér v interspecifickém křížení u rodu Cucumis. Pylová zrna C. sativus a C. melo byla izolována různými metodami. Byla použita řada centrifugačních metod a metoda přímá. Byly využity tři různé izolační roztoky (NLN, YST, VB) a dvě kultivační média pro izolovaná pylová zrna (C. sativus, C. melo) a vajíčka (C. sativus) (YS a CP). Nejvyšší stupeň regenerace, kterého bylo dosaženo, byl mikrokalus.

Type

D - Article in proceedings

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/QF4108" target="_blank" >QF4108: Utilization of protoplast fusion technique in breeding of the important cultivated crops of the genera Brassica, Cucumis and Solanum.</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Cucurbitaceae 2008: Proceedings of the IXth EUCARPIA Meeting on Genetics and Breeding Cucurbitaceae

ISBN

978-2-7380-1251-7

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

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Publisher name

INRA

Place of publication

Avignon, France

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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