Genetic and biological diversity of Pea seed-borne mosaic virus isolates occuring in the Czech Republic.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005262" target="_blank" >RIV/61989592:15310/08:00005262 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Genetic and biological diversity of Pea seed-borne mosaic virus isolates occuring in the Czech Republic.
Original language description
Eight isolates of the Pea seed-borne mosaic virus (PSbMV) front the Czech Republic were studied regarding their biological and molecular characteristics. Molecular characterization using RT-PCR was done on the 5'(Nter-)NIb-CP-UTR3' region amplified usinguniversal CPUP/P9502 primer pair and the newly designed PSB8812/PSB944, and PSB8800/PSB9440 primer pairs, respectively. Sequential and phylogenetic analysis of CP-UTR3' region from all isolates showed that (lie available Czech and GenBank PSbMV isolateswere distributed into 4 clusters in agreement with their diversification and according to their biological characteristics (i.e. pathotype). The molecular data were confirmed by biological testing on different pea cultivars. The Czech isolates were distributed into two pathotypes, the P-1 (7 isolates) and P-4 (1 isolate).
Czech name
Genetická a biologická variabilita viru semenem přenosné mozaiky hrachu v České republice.
Czech description
Osm izolátů PSbMV pocházejících z České republiky bylo charakterizováno na molekulární a biologické úrovni. Sekvenční a fylogenetická analýza CP-UTR oblasti všech izolátů ukázala jejich příslušnost k PSbMV, a jejich distribuci do čtyř nezávislých skupin.Molekulární data byla potvrzena v biologických testech pomocí diferenciačních inií. České izoláty vykazovaly příslušnost ke dvěma biologickým patotypům, patotypu P-1 (sedm izolátů) a patotypu P-4 (1 izolát).
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QF3072" target="_blank" >QF3072: Development of transgenic pea (Pisum sativum L.) lines with enhanced resistance to viral pathogens.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Virologica
ISSN
0001-723X
e-ISSN
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Volume of the periodical
52
Issue of the periodical within the volume
1
Country of publishing house
SK - SLOVAKIA
Number of pages
5
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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