Quality Control of Photosystem II: Reactive Oxygen Species are Responsible for the Damage to Photosystem II under Moderate Heat Stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005291" target="_blank" >RIV/61989592:15310/08:00005291 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Quality Control of Photosystem II: Reactive Oxygen Species are Responsible for the Damage to Photosystem II under Moderate Heat Stress

Original language description

Moderate heat stress (40 °C for 30 min) on spinach thylakoid membranes induced cleavage of the reaction center-binding D1 protein of photosystem II, aggregation of the D1 protein with the neighboring polypeptides D2 and CP43, and release of three extrinsic proteins, PsbO, -P, and -Q. These heat-induced events were suppressed under anaerobic conditions or by the addition of sodium ascorbate, a general scavenger of reactive oxygen species. In accordance with this, singlet oxygen and hydroxyl radicals weredetected in spinach photosystem II membranes incubated at 40 °C for 30 min with electron paramagnetic resonance spin-trapping spectroscopy. The moderate heat stress also induced significant lipid peroxidation under aerobic conditions. We suggest that the reactive oxygen species are generated by heat-induced inactivation of a water-oxidizing manganese complex and through lipid peroxidation. Although occurring in the dark, the damages caused by the moderate heat stress to photosystem II a

Czech name

Kvalitní kontrola Fotosystému II: Reaktivní formy kyslíku jsou odpovědné za poškození Fotosystému II při působení teplotního stresu

Czech description

Teplotní stres (40 °C for 30 min) thylakoidních membrán izolovaných ze špenátu způsobil rozpad D1 proteinu reakčního centra fotosystému II, agregaci D1 proteinu s okolními proteiny D2 a CP43 a uvolnění tří vnějších proteinů PsbO, -P, a -Q.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA522%2F06%2F0979" target="_blank" >GA522/06/0979: Mechanisms of plant resistance against ROS induced in photosynthetic apparatus by a photoinhibitory stress</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Volume of the periodical

283

Issue of the periodical within the volume

42

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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