Lamin A/C and polymeric actin in genome organization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005496" target="_blank" >RIV/61989592:15310/08:00005496 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/08:00318998 RIV/00216224:14330/08:00026234
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Lamin A/C and polymeric actin in genome organization
Original language description
In this work, we have studied the structural and functional linkage between lamin A/C, nuclear actin, and organization of chromosome territories (CTs) in mammary carcinoma MCF-7 cells. Selective down-regulation of lamin A/C expression led to disruption of the lamin A/C perinuclear layer and disorganization of lamin-bound emerin complexes at the inner nuclear membrane. The silencing of lamin A/C expression resulted in a decrease in the volume and surface area of chromosome territories, especially in chromosomes with high heterochromatin content. Inhibition of actin polymerization led to relaxation of the structure of chromosome territories, and an increase in the volumes and surface areas of the chromosome territories of human chromosomes 1, 2 and 13. The results show an important role of polymeric actin in the organization of the nuclei and the chromosome territories.
Czech name
Lamin A/C a polymerní aktin v organizaci genomu
Czech description
V této práci jsme studovali strukrní a funkční spojení mezi laminem A/C, polymerním aktinem a organizací chromozómových teritorií v buňkách MCF-7. Selektivní umlčení exprese laminu A/C vedlo desintegraci vrstvy laminu A/C na okraji jádra a také komplex emerinu vážícího se na lamin na vnitřní straně jaderné membrány. Toto umlčení vedlo ke snížení objemu a povrchu chromoz´mových teritorií, hlavně těch s vyšším obsahem heterochromatinu. Inhibice polymerizace aktinu se projevila relaxací chromozómových teritorií chromozómů 1, 2 a 13 a vedla ke zvýšení jejich objemů a povrchů.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecules and Cells
ISSN
1016-8478
e-ISSN
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Volume of the periodical
26
Issue of the periodical within the volume
4
Country of publishing house
KR - KOREA, REPUBLIC OF
Number of pages
6
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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