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Spatial and temporal changes in endogenous cytokinins in developing pea roots

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005515" target="_blank" >RIV/61989592:15310/08:00005515 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389030:_____/08:00322260

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Spatial and temporal changes in endogenous cytokinins in developing pea roots

  • Original language description

    Germination and seedling establishment follows a distinct pattern which is partly controlled by hormones. Roots have high levels of cytokinins. By quantifying the fluctuations in endogenous cytokinins over time, further insight may be gained into the role of cytokinins during germination and seedling establishment. Radicles were excised from sterile Pisum sativum L. seeds after 30 min and 5 h imbibition. Seedlings germinated on agar were harvested after 1, 3, 6 and 9 days. The roots were divided into the root tip, root free zone, secondary root zone and from day 6, the secondary roots. Samples were purified by various chromatographic methods and endogenous cytokinins detected by LC(+)ES-MS. Benzyladenine levels doubled after 5 h imbibition and then gradually decreased over time. Low concentrations of cis-Zeatin (cZ) type cytokinins were detected in the radicle after 30 min imbibition. After 5 h imbibition, cis-zeatin riboside-5'-monophosphate had greatly increased. The total cytokinin

  • Czech name

    Prostorové a časové změny u endogenních cytokininů ve vyvíjejících se kořenech hrachu

  • Czech description

    Studium hladin endogenních cytokininů a popis jejich kolísání v časovém průběhu ukázaly na možnou roli cytokininů během klíčení a ujmutí semenáčků Pisum sativum L. Kořeny byly rozděleny na různé časti a v těch byly stanovovány endogenní cytokininy. Celkový obsah cytokininů se zvyšoval a ribotidy byly převládající formou. Byla studována i biosyntéza isopentenyladeninu a zeatinu. Kolísání koncentrací u různych forem cytokininů ukazují, že v kořenech hrachu mohou být syntetizovány z různých drah.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EF - Botany

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Planta

  • ISSN

    0032-0935

  • e-ISSN

  • Volume of the periodical

    227

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    11

  • Pages from-to

  • UT code for WoS article

  • EID of the result in the Scopus database