The heterochromatin as a marker for protoplast differentiation of Cucumis sativus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005540" target="_blank" >RIV/61989592:15310/08:00005540 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/09:00010196
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The heterochromatin as a marker for protoplast differentiation of Cucumis sativus
Original language description
The protoplast cultures of Cucumis sativus in two culture systems were used to study heterochromatin reassembly during dedifferentiation of isolated protoplasts and their subsequent differentiation into calli and proembryos. Here we show that dedifferantiation of the cucumber mesophyll cells is accompanied by a dramatic reduction in size and numbers of nuclear chromocenters. Although chromocenters were newly established during protoplast culture, the measured relative heterochromatin content differed according to the culture system used. Protoplast culture leading to proembryo formation displayed a lower level of relative heterochromatin content than cultures resulting in calli and the relative heterochromatin content reached values close to those estimated for somatic embryos.
Czech name
Heterochromatin jako marker diferenciace protoplastů Cucumis sativus
Czech description
Reorganizace protoplastů Cucumis sativus ve dvou kultivačních systémech byla studována v rámci dediferenciace izolovaných protoplastů a následné diferenciaci v kalus a proembrya. Ukázali jsme zde, že dediferenciace mezofylových buněk je doprovázena dramatickou redukcí počtu a velikostí jaderných chromocenter. Ačkoliv jsou chromocentra během diferenciace znovu ustanovena, liší se velikost relativního obsahu heterochromatinu v jádrech kultur vzhledem ke kultivačnímu systému. V systému, kde protoplastovy diferenciují v proembrya je obsah heterochromatinu nižší než v kulturách vedoucí k produkci kalusu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell, Tissue and Organ Culture (online)
ISSN
1573-5044
e-ISSN
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Volume of the periodical
29
Issue of the periodical within the volume
97
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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