High Throughput Multiplexed Method for Evaluation of Enantioselective Performance of Chiral Selectors by HPLC-ESI-MS and Dynamic Titration: Cinchona Alkaloid Carbamates Discriminating N-blocked Amino Acids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010417" target="_blank" >RIV/61989592:15310/09:00010417 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

High Throughput Multiplexed Method for Evaluation of Enantioselective Performance of Chiral Selectors by HPLC-ESI-MS and Dynamic Titration: Cinchona Alkaloid Carbamates Discriminating N-blocked Amino Acids

Original language description

Because of the generally different interaction of enantiomers with biological systems, there has been an ever increasing demand for artificial highly enantioselective systems that can facilitate separation processes involved in the research and development of enantiomerically pure drugs. Such systems may be discovered by large-scale screening of compound libraries which warrants rapid and cost-efficient screening methods. Here, we demonstrate enantioselectivity determination for systems of cinchona alkaloid carbamates and N-blocked amino acids using HPLC-MS and the recently developed dynamic titration technique (Frycak P, Schug KA. Anal Chem 2008;80:13851393). A mixture of nine N-blocked amino acids (either D or L enantiomers) was separated on a reversed-phase column with cinchona alkaloid carbamates added post-column. Dissociation constants of the observed noncovalent complexes were determined from the HPLC-MS data. Enantioselectivity was then calculated from the dissociation constant

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chirality

ISSN

0899-0042

e-ISSN

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Volume of the periodical

21

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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