Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010627" target="_blank" >RIV/61989592:15310/09:00010627 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/09:00328264

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium

Original language description

Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens. Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin(IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta-Topolin riboside (mTR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when mTR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Boron was toxic at higher (0.4 and 0.5 mM) concentrations. Plantlets rooted readily in half-strength cytokinin-free MS media supplemented with 2.5 mu M IAA. Rooted

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

<a href="/en/project/GA522%2F06%2F0108" target="_blank" >GA522/06/0108: New cytokinin derivatives in plants: isolation, identification and biological activity</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

South African Journal of Botany

ISSN

0254-6299

e-ISSN

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Volume of the periodical

75

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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