Expression and activity of vitamin D receptor in the human placenta and in choriocarcinoma BeWo and JEG-3 cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010863" target="_blank" >RIV/61989592:15310/09:00010863 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/09:00210148
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Expression and activity of vitamin D receptor in the human placenta and in choriocarcinoma BeWo and JEG-3 cell lines
Original language description
VitaminDreceptor (VDR) regulates the expression ofmany genes involved in mineralmetabolism, cellular proliferation, differentiation and drug biotransformation. We studied the expression and activity of VDR and its heterodimerization partner retinoid X receptor-alfa (RXRalfa) in choriocarcinoma trophoblast cell lines BeWo and JEG-3, in comparison with human isolated placental cytotrophoblasts and human full term placenta. We found that VDR and RXRalfa are localised in the human term placenta trophoblastand expressed in isolated cytotrophoblasts. However, we found low expression and no transcriptional activity of VDR in used choriocarcinoma cell lines. The inhibitor of DNA methylation, 5-deoxy-3´-azacytidine, and histone deacetylase inhibitor sodium butyrate partially restored the expression of VDR, suggesting an epigenetic suppression of the gene in choriocarcinoma cells. Differentiation ofBeWo cells resulted in up-regulation of VDRmRNA. Finally,we observed a non-genomic effect of 1,25
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FB - Endocrinology, diabetology, metabolism, nutrition
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA303%2F07%2F0128" target="_blank" >GA303/07/0128: Study on tissue-specific aspects of transcriptional regulation of selected P-450 enzymes and drug transporters.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular and Cellular Endocrinology
ISSN
0303-7207
e-ISSN
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Volume of the periodical
299
Issue of the periodical within the volume
2
Country of publishing house
IE - IRELAND
Number of pages
10
Pages from-to
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UT code for WoS article
000263662500006
EID of the result in the Scopus database
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