Prussian Blue acts as a mediator in a reagentless cytokinin biosensor.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F11%3A33118639" target="_blank" >RIV/61989592:15310/11:33118639 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2011.06.018" target="_blank" >http://dx.doi.org/10.1016/j.aca.2011.06.018</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2011.06.018" target="_blank" >10.1016/j.aca.2011.06.018</a>
Alternative languages
Result language
angličtina
Original language name
Prussian Blue acts as a mediator in a reagentless cytokinin biosensor.
Original language description
An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzesan irreversible cleavage of N6-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED0007%2F01%2F01" target="_blank" >ED0007/01/01: Centre of the Region Haná for Biotechnological and Agricultural Research</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
701
Issue of the periodical within the volume
701
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
218-223
UT code for WoS article
000294144400015
EID of the result in the Scopus database
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