The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33118951" target="_blank" >RIV/61989592:15310/12:33118951 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s11240-011-0035-3" target="_blank" >http://dx.doi.org/10.1007/s11240-011-0035-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-011-0035-3" target="_blank" >10.1007/s11240-011-0035-3</a>

Result language

angličtina

Original language name

The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts

Original language description

The present study investigated changes in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in isolated mesophyll protoplasts and cell cultures of the cucumber Cucumis sativus cv. Marketer. Although only a minor increasein the level of nitrogen oxide (NO) was observed during the first 7 days of culture following protoplast isolation, a substantial accumulation of ROS was detected. Compounds known to modulate endogenous ROS and RNS levels were employed to study their role in cucumber protoplast regeneration and growth. Supplementing the culture medium with the NO donors S-nitrosoglutathione and sodium nitroprusside and the ROS scavenger ascorbate significantly increased protoplast viability and cell density. In contrast, cell density was significantly decreased following the addition of catalase to the medium. Scavenging of ROS and RNS induced the formation of cucumber microcalli, thus suggesting a differential role of NO in the maintenance of cell via

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/ME08048" target="_blank" >ME08048: The role of nitric oxide in signal pathways during plant development and stress responses.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell, Tissue and Organ Culture (print)

ISSN

0167-6857

e-ISSN

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Volume of the periodical

108

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

237-249

UT code for WoS article

000299329500007

EID of the result in the Scopus database

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