Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33141796" target="_blank" >RIV/61989592:15310/12:33141796 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2147/IJN.S28237" target="_blank" >http://dx.doi.org/10.2147/IJN.S28237</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2147/IJN.S28237" target="_blank" >10.2147/IJN.S28237</a>

Result language
angličtina
Original language name
Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles
Original language description
Novel superparamagnetic surface-active maghemite nanoparticles (SAMNs) characterized by a diameter of 10 +/- 2 nm were modified with bovine serum amine oxidase, which used rhodamine B isothiocyanate (RITC) adduct as a fluorescent spacer-arm. A fluorescent and magnetically drivable adduct comprised of bovine serum copper-containing amine oxidase (SAMN-RITC-BSAO) that immobilized on the surface of specifically functionalized magnetic nanoparticles was developed. The multifunctional nanomaterial was characterized using transmission electron microscopy, infrared spectroscopy, mass spectrometry, and activity measurements. The results of this study demonstrated that bare magnetic nanoparticles form stable colloidal suspensions in aqueous solutions. The maximum binding capacity of bovine serum amine oxidase was approximately 6.4 mg g(-1) nanoparticles. The immobilization procedure reduced the catalytic activity of the native enzyme to 30% +/- 10% and the Michaelis constant was increased by a
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Project
<a href="/en/project/ED2.1.00%2F03.0058" target="_blank" >ED2.1.00/03.0058: Regional Centre of Advanced Technologies and Materials</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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