Rifampicin Does not Significantly Affect the Expression of Small Heterodimer Partner in Primary Human Hepatocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142723" target="_blank" >RIV/61989592:15310/12:33142723 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/12:10124924

Result on the web

<a href="http://www.frontiersin.org/Drug_Metabolism_and_Transport/10.3389/fphar.2012.00001/full" target="_blank" >http://www.frontiersin.org/Drug_Metabolism_and_Transport/10.3389/fphar.2012.00001/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphar.2012.00001" target="_blank" >10.3389/fphar.2012.00001</a>

Result language

angličtina

Original language name

Rifampicin Does not Significantly Affect the Expression of Small Heterodimer Partner in Primary Human Hepatocytes

Original language description

The small/short heterodimer partner (SHP, NR0B2) is a nuclear receptor corepressor lacking a DNA binding domain. SHP is induced by bile acid-activated farnesoid X receptor (FXR) resulting in CYP7A1 gene suppression. In contrast, Pregnane X receptor (PXR)activation by its ligands was recently suggested to inhibit SHP gene transactivation to maximize the induction of PXR target genes. However, there are also conflicting reports in literature whether PXR or rodent Pxr activation down-regulates SHP/Shp expression. Moreover, the PXR-mediated regulation of the SHP gene has been studied only at the SHP mRNA and transactivation (gene reporter assay) levels. In this study, we studied the effect of rifampicin, a prototype PXR ligand, on SHP mRNA, and protein expression in three primary human hepatocyte cultures. We found that SHP mRNA is not systematically down-regulated in hepatocyte in culture after 24 h treatment with rifampicin. Consistently, we did not observe down-regulation of SHP protei

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

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Project

<a href="/en/project/GA303%2F07%2F0128" target="_blank" >GA303/07/0128: Study on tissue-specific aspects of transcriptional regulation of selected P-450 enzymes and drug transporters.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Pharmacology

ISSN

1663-9812

e-ISSN

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Volume of the periodical

3

Issue of the periodical within the volume

Leden 2012

Country of publishing house

CH - SWITZERLAND

Number of pages

5

Pages from-to

1-5

UT code for WoS article

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EID of the result in the Scopus database

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