Structural Characterization of Nitrosomonas europaea Cytochrome c-552 Variants with Marked Differences in Electronic Structure

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33145383" target="_blank" >RIV/61989592:15310/13:33145383 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/cbic.201300118" target="_blank" >http://dx.doi.org/10.1002/cbic.201300118</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cbic.201300118" target="_blank" >10.1002/cbic.201300118</a>

Result language

angličtina

Original language name

Structural Characterization of Nitrosomonas europaea Cytochrome c-552 Variants with Marked Differences in Electronic Structure

Original language description

Nitrosomonas europaea cytochrome c-552 (Ne c-552) variants with the same His/Met axial ligand set but with different EPR spectra have been characterized structurally, to aid understanding of how molecular structure determines heme electronic structure. Visible light absorption, Raman, and resonance Raman spectroscopy of the protein crystals was performed along with structure determination. The structures solved are those of Ne c-552, which displays a "HALS" (or highly anisotropic low-spin) EPR spectrum,and of the deletion mutant Ne N64delta, which has a rhombic EPR spectrum. Two X-ray crystal structures of wild-type Ne c-552 are reported; one is of the protein isolated from N. europaea cells (Ne c-552n, 2.35 ? resolution), and the other is of recombinant protein expressed in Escherichia coli (Ne c-552r, 1.63 ? resolution). Ne N64delta crystallized in two different space groups, and two structures are reported [monoclinic (2.1 ? resolution) and hexagonal (2.3 ? resolution)]. Comparison

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/ED2.1.00%2F03.0058" target="_blank" >ED2.1.00/03.0058: Regional Centre of Advanced Technologies and Materials</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ChemBioChem

ISSN

1439-4227

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

14

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

"1828?1838"

UT code for WoS article

000325851800018

EID of the result in the Scopus database

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