Glucocorticoid receptor regulates organic cation transporter 1 (OCT1, SLC22A1) expression via HNF4alfa upregulation in primary human hepatocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33147782" target="_blank" >RIV/61989592:15310/13:33147782 - isvavai.cz</a>

Result on the web

<a href="http://www.if-pan.krakow.pl/pjp/pdf/2013/5_1322.pdf" target="_blank" >http://www.if-pan.krakow.pl/pjp/pdf/2013/5_1322.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Glucocorticoid receptor regulates organic cation transporter 1 (OCT1, SLC22A1) expression via HNF4alfa upregulation in primary human hepatocytes

Original language description

Organic cation transporter 1 (OCT1, SLC22A1) is a membrane transporter that is important for therapeutic effect of the antidiabetic drug metformin. Its liver-specific expression in hepatocytes is strongly controlled by hepatocyte nuclear factor-4alfa (HNF4alfa). HNF4alfa expression and transcriptional activity have been demonstrated to be augmented by glucocorticoid receptor (GR) in human hepatocytes and rodent livers. It was examined whether GR activation indirectly induces OCT1 gene expression via HNF4alfa up-regulation in primary human hepatocytes. We also examined which other transcription factors are involved in OCT1 gene expression and whether they are regulated by dexamethasone using qRT-PCR and gene reporter assays. RESULTS: We found that dexamethasone significantly up-regulates OCT1 mRNA and protein in normal primary human hepatocytes, but not in hepatocyte-derived tumor cell lines HepG2 and MZ-Hep1. Consistently, we observed that HNF4alfa is induced by dexamethasone in primar

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pharmacological Reports

ISSN

1734-1140

e-ISSN

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Volume of the periodical

65

Issue of the periodical within the volume

5

Country of publishing house

PL - POLAND

Number of pages

14

Pages from-to

1322-1335

UT code for WoS article

000330815700028

EID of the result in the Scopus database

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