Analysis of gibberellins as free acids by ultra performance liquid chromatography-tandem mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33148292" target="_blank" >RIV/61989592:15310/13:33148292 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/13:00399103
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S003991401300218X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S003991401300218X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2013.03.068" target="_blank" >10.1016/j.talanta.2013.03.068</a>

Result language
angličtina
Original language name
Analysis of gibberellins as free acids by ultra performance liquid chromatography-tandem mass spectrometry
Original language description
A robust, reliable and high-throughput method for extraction and purification of gibberellins (GAs), a group of tetracyclic diterpenoid carboxylic acids that include endogenous growth hormones, from plant material was developed. The procedure consists oftwo solid-phase extraction steps (Oasis (R) MCX-HLB and Oasis (R) MAX) and gives selective enrichment and efficient clean-up of these compounds from complex plant extracts. The method was tested with plant extracts of Brassica napus and Arabidopsis thaliana, from which total recovery of internal standards of about 72% was achieved. A rapid baseline chromatographic separation of 20 non-derivatised GAs by ultra performance liquid chromatography is also presented where a reversed-phase chromatographic column Acquity CSH (R) and a mobile phase consisting of methanol and aqueous 10 mM-ammonium formate is used. This method enables sensitive and precise quantitation of GAs by MS/MS in multiple-reaction monitoring mode (MRM) by a standard isot
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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