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Dynamics and Organization of Cortical Microtubules as Revealed by Superresolution Structured Illumination Microscopy

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151254" target="_blank" >RIV/61989592:15310/14:33151254 - isvavai.cz</a>

  • Alternative codes found

    RIV/61989592:15110/14:33151254

  • Result on the web

    <a href="http://www.plantphysiol.org/content/165/1/129.long" target="_blank" >http://www.plantphysiol.org/content/165/1/129.long</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1104/pp.114.238477" target="_blank" >10.1104/pp.114.238477</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Dynamics and Organization of Cortical Microtubules as Revealed by Superresolution Structured Illumination Microscopy

  • Original language description

    Plants employ acentrosomal mechanisms to organize cortical microtubule arrays essential for cell growth and differentiation. Using structured illumination microscopy (SIM) adopted for the optimal documentation of Arabidopsis (Arabidopsis thaliana) hypocotyl epidermal cells, dynamic cortical microtubules labeled with green fluorescent protein fused to the microtubule-binding domain of the mammalian microtubule-associated protein MAP4 and with green fluorescent protein-fused to the alpha tubulin6 were comparatively recorded in wild-type Arabidopsis plants and in the mitogen-activated protein kinase mutant mpk4 possessing the former microtubule marker. The mpk4 mutant exhibits extensive microtubule bundling, due to increased abundance and reduced phosphorylation of the microtubule-associated protein MAP65-1, thus providing a very useful genetic tool to record intrabundle microtubule dynamics at the subdiffraction level. SIM imaging revealed nano-sized defects in microtubule bundling, spat

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Plant Physiology

  • ISSN

    0032-0889

  • e-ISSN

  • Volume of the periodical

    165

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    20

  • Pages from-to

    129-148

  • UT code for WoS article

    000335906300011

  • EID of the result in the Scopus database