Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151289" target="_blank" >RIV/61989592:15310/14:33151289 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/protocol/10.1007%2F978-1-4939-1420-3_20" target="_blank" >http://link.springer.com/protocol/10.1007%2F978-1-4939-1420-3_20</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-1420-3_20" target="_blank" >10.1007/978-1-4939-1420-3_20</a>

Result language
angličtina
Original language name
Integrative Chemical Proteomics and Cell Biology Methods to Study Endocytosis and Vesicular Trafficking in Arabidopsis
Original language description
We present a comprehensive approach combining proteomics and cell biology to study vesicular trafficking in plants. Within this approach, we exploit chemical compounds inhibiting particular vesicular trafficking events in plant cells. Treatment of plantswith these relatively specific inhibitors results in intracellular accumulation of proteins being transported by vesicles as well as in a change in abundance of regulatory proteins. Such pharmacological inhibition allows for identification of key proteins, and for further detailed functional investigation using cell biological, molecular biological, and biochemical methods used for validation of proteomic results.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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