Bulk derivatization and direct injection of human cerebrospinal fluid for trace-level quantification of endogenous estrogens using trap-and-elute liquid chromatography with tandem mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151656" target="_blank" >RIV/61989592:15310/14:33151656 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400165/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400165/pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201400165" target="_blank" >10.1002/jssc.201400165</a>

Result language
angličtina
Original language name
Bulk derivatization and direct injection of human cerebrospinal fluid for trace-level quantification of endogenous estrogens using trap-and-elute liquid chromatography with tandem mass spectrometry
Original language description
Although there are existing methods for determining estrogen in human bodily fluids including blood plasma and serum, very little information is available regarding estrogen levels in human cerebrospinal fluid (CSF), which is critical to assess in studies of neuroprotective functions and diffusion of neuroprotective estrogens across the blood-brain barrier. To address this problem, a liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of four endogenous estrogens (estrone, 17 alpha-estradiol, 17 alpha-estradiol, and estriol) in human CSF was developed. An aliquot (300 mu L) of human CSF was bulk derivatized using dansyl chloride in the sample and 10 mu L was directly injected onto a restricted-access media trap column for protein removal. No off-line sample extraction or cleanup was needed. The limits of detection of estrone, 17 alpha-estradiol, 17 beta-estradiol, and estriol were 17, 28, 13, and 30 pg/mL, respectively, which is in the parts-p
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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