Retention behavior of a homologous series and positional isomers of aliphatic amino acids in hydrophilic interaction chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151680" target="_blank" >RIV/61989592:15310/14:33151680 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/14:10282195

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201301348/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201301348/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201301348" target="_blank" >10.1002/jssc.201301348</a>

Result language

angličtina

Original language name

Retention behavior of a homologous series and positional isomers of aliphatic amino acids in hydrophilic interaction chromatography

Original language description

The retention behavior of several series of free - and -amino acids and positional isomers of amino pentanoic acid in the hydrophilic interaction chromatography mode (HILIC) was studied. The study was carried out on three stationary phases followed by post-column derivatization with fluorescence detection in order to describe the retention mechanism of the tested amino acids. The effect of chromatographic conditions including acetonitrile content in the mobile phase, mobile phase pH (ranging from 3.5 to6.5) and concentration of buffer in the mobile phase was investigated. The effect of the number of carbon atoms (n(C)) in aliphatic chains of the individual homologue of - and -amino acids and the logarithm of the partition coefficient (logD) on retention was also a part of the presented study. A good correlation (r } 0.98) between the logk and logD values of amino acids or n(C), respectively, was observed. The described linear relationships were subsequently applied to predict the rete

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

739-747

UT code for WoS article

000333538600001

EID of the result in the Scopus database

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