Proteomic identification of a candidate sequence of wheat cytokinin-binding protein 1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151716" target="_blank" >RIV/61989592:15310/14:33151716 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs00344-014-9419-z" target="_blank" >http://link.springer.com/article/10.1007%2Fs00344-014-9419-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00344-014-9419-z" target="_blank" >10.1007/s00344-014-9419-z</a>

Result language

angličtina

Original language name

Proteomic identification of a candidate sequence of wheat cytokinin-binding protein 1

Original language description

Our knowledge on the principle mechanisms of cytokinin action has been significantly deepened over the last years, but several weakly explored areas still remain on the map of cytokinin cellular physiology. Cytokinin-binding proteins could also be included in this pending field of cytokinin research. Probably, the best explored representative of this group is the wheat cytokinin-binding protein 1 (CBP-1). The role of this germ-allocated protein as a presumable regulator of free aromatic cytokinin levelsduring grain germination has been discussed intensively. To dig deeper into this interesting protein, this study was aimed at the identification of the CBP-1 amino acid sequence. A combination of in silico BLAST search, classical biochemical CBP-1 purification based on isoelectric point precipitation and ion-exchange chromatography, and proteomic analysis of the isolated protein by ultra-high resolution tandem mass spectrometry allowed us to uncover and validate two CBP-1 subunit candid

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Plant Growth Regulation

ISSN

0721-7595

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

896-902

UT code for WoS article

000344917800018

EID of the result in the Scopus database

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