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Proteomic analysis of barley cell nuclei purified by flow sorting

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151775" target="_blank" >RIV/61989592:15310/14:33151775 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389030:_____/14:00433901

  • Result on the web

    <a href="http://www.karger.com/Article/FullText/365311" target="_blank" >http://www.karger.com/Article/FullText/365311</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1159/000365311" target="_blank" >10.1159/000365311</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Proteomic analysis of barley cell nuclei purified by flow sorting

  • Original language description

    Many proteins are present in the nucleus; some are involved with its structural and functional organization, some with gene expression, and some with cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both the artifactual alteration of the proteins and the extent of contamination with non-nuclear proteins. The conventional multi-step fractionation procedure is both laborious and prone to contamination. Here, we describe asingle-step method based on flow sorting. The method allows the separation of G1, S and G2 phase nuclei and minimizes the risk of contamination by non-nuclear proteins. Preliminary results obtained using G1 phase cell nuclei from barley root tips indicate that flow sorting coupled with a protein/peptide separation and mass spectrometry will permit a comprehensive characterization of the plant nuclear proteome.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Cytogenetic and Genome Research

  • ISSN

    1424-8581

  • e-ISSN

  • Volume of the periodical

    143

  • Issue of the periodical within the volume

    1-3

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    9

  • Pages from-to

    78-86

  • UT code for WoS article

    000342614100008

  • EID of the result in the Scopus database