Proteomic analysis of barley cell nuclei purified by flow sorting
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151775" target="_blank" >RIV/61989592:15310/14:33151775 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/14:00433901
Result on the web
<a href="http://www.karger.com/Article/FullText/365311" target="_blank" >http://www.karger.com/Article/FullText/365311</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1159/000365311" target="_blank" >10.1159/000365311</a>
Alternative languages
Result language
angličtina
Original language name
Proteomic analysis of barley cell nuclei purified by flow sorting
Original language description
Many proteins are present in the nucleus; some are involved with its structural and functional organization, some with gene expression, and some with cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both the artifactual alteration of the proteins and the extent of contamination with non-nuclear proteins. The conventional multi-step fractionation procedure is both laborious and prone to contamination. Here, we describe asingle-step method based on flow sorting. The method allows the separation of G1, S and G2 phase nuclei and minimizes the risk of contamination by non-nuclear proteins. Preliminary results obtained using G1 phase cell nuclei from barley root tips indicate that flow sorting coupled with a protein/peptide separation and mass spectrometry will permit a comprehensive characterization of the plant nuclear proteome.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytogenetic and Genome Research
ISSN
1424-8581
e-ISSN
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Volume of the periodical
143
Issue of the periodical within the volume
1-3
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
78-86
UT code for WoS article
000342614100008
EID of the result in the Scopus database
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