Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33153248" target="_blank" >RIV/61989592:15310/14:33153248 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-0922-3_2" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-0922-3_2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-0922-3_2" target="_blank" >10.1007/978-1-4939-0922-3_2</a>
Alternative languages
Result language
angličtina
Original language name
Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR
Original language description
Although it is generally accepted that signal transduction in plant mitogen-activated protein kinase signaling cascades is regulated via rapid posttranslational modifications, there are also several compelling examples of swift stress induced transcriptional activation of plant MAP kinase genes. A possible function of these fast and transient events is to compensate for protein losses caused by degradation of phosphorylated MAP kinases within stimulated pathways. Nevertheless, there is still need for additional evidence to precisely describe the regulatory role of plant MAP kinase transcriptional dynamics, especially in the context of whole stress stimulated pathways including also other signaling molecules and transcription factors. During the last two decades a reverse transcription quantitative real-time PCR became a golden choice for the accurate and fast quantification of the gene expression and gene expression dynamic. In here, we provide a robust, cost-effective SYBR Green-based
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Plant MAP Kinases: Methods and Protocols
ISBN
978-1-4939-0921-6
Number of pages of the result
13
Pages from-to
13-25
Number of pages of the book
266
Publisher name
Humana Press
Place of publication
Clifton
UT code for WoS chapter
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