Effects of sulforaphane and its S- and R-enantiomers on the expression and activities of human drug-metabolizing cytochromes P450

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33155401" target="_blank" >RIV/61989592:15310/15:33155401 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/15:33155401
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S1756464615000687" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1756464615000687</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jff.2015.02.006" target="_blank" >10.1016/j.jff.2015.02.006</a>

Result language
angličtina
Original language name
Effects of sulforaphane and its S- and R-enantiomers on the expression and activities of human drug-metabolizing cytochromes P450
Original language description
The effects of S/R-enantiomers of sulforaphane on drug-metabolizing cytochromes P450, in particular: (i) expression of CYP1A1/2, CYP2B6, CYP2C9, CYP3A4 mRNAs and proteins in human hepatocytes; (ii) mRNA expression and activity of pregnane X receptor (PXR), aryl hydrocarbon receptor (AhR) and glucocorticoid receptor (GR) in human hepatocytes and transgenic gene reporter cell lines, respectively; (iii) catalytic activity of CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and CYP3A4 in human liver microsomes and human hepatocytes were investigated. The major findings are that (i) sulforaphane exerted insignificant effects on the expression of CYP1A1/2, CYP2B6, CYP2C9, CYP3A4 mRNAs and proteins. We observed induction of CYP2C9 and CYP2B6, but these effects were inconsistent, not enantiospecific, with inter-individual variability between human hepatocytes cultures; sulforaphane displayed a dose-dependent but not enantiospecific cytotoxicity in HepG2, HeLa and LS174T cells. S
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
<a href="/en/project/GBP303%2F12%2FG163" target="_blank" >GBP303/12/G163: Centre of drug-dietary supplements interactions and nutrigenetics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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