Preparation of plants for developmental and cellular imaging by light-sheet microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33155769" target="_blank" >RIV/61989592:15310/15:33155769 - isvavai.cz</a>

Result on the web

<a href="http://www.nature.com/nprot/journal/v10/n8/full/nprot.2015.081.html" target="_blank" >http://www.nature.com/nprot/journal/v10/n8/full/nprot.2015.081.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nprot.2015.081" target="_blank" >10.1038/nprot.2015.081</a>

Result language

angličtina

Original language name

Preparation of plants for developmental and cellular imaging by light-sheet microscopy

Original language description

Long-term fluorescence live-cell imaging experiments have long been limited by the effects of excitation-induced phototoxicity. The advent of light-sheet microscopy now allows users to overcome this limitation by restricting excitation to a narrow illumination plane. In addition, light-sheet imaging allows for high-speed image acquisition with uniform illumination of samples composed of multiple cell layers. The majority of studies conducted thus far have used custom-built platforms with specialized hardware and software, along with specific sample handling approaches. The first versatile commercially available light-sheet microscope, Lightsheet Z.1, offers a number of innovative solutions, but it requires specific strategies for sample handling duringlong-term imaging experiments. There are currently no standard procedures describing the preparation of plant specimens for imaging with the Lightsheet Z.1. Here we describe a detailed protocol to prepare plant specimens for light-sheet

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Protocols

ISSN

1754-2189

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

1234-1247

UT code for WoS article

000358482200008

EID of the result in the Scopus database

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