Endosomal interactions during root hair growth
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33156449" target="_blank" >RIV/61989592:15310/16:33156449 - isvavai.cz</a>
Result on the web
<a href="http://journal.frontiersin.org/article/10.3389/fpls.2015.01262/full" target="_blank" >http://journal.frontiersin.org/article/10.3389/fpls.2015.01262/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2015.01262" target="_blank" >10.3389/fpls.2015.01262</a>
Alternative languages
Result language
angličtina
Original language name
Endosomal interactions during root hair growth
Original language description
The dynamic localization of endosomal compartments labeled with targeted fluorescent protein tags is routinely followed by time lapse fluorescence microscopy approaches and single particle tracking algorithms. In this way trajectories of individual endosomes can be mapped and linked to physiological processes as cell growth. However, other aspects of dynamic behavior including endosomal interactions are difficult to follow in this manner. Therefore we characterized the localization and dynamic properties of early and late endosomes throughout the entire course of root hair formation by means of spinning disc time lapse imaging and post-acquisition automated multitracking and quantitative analysis. Detailed data analysis revealed a particular transient interaction between late endosomes - termed herein as dancing-endosomes - which is not concluding to vesicular fusion. Endosomes preferentially located in the root hair tip interacted as dancing-endosomes and traveled short distances during this interaction. Finally, sizes of early and late endosomes were addressed by means of super-resolution structured illumination microscopy (SIM) to corroborate measurements on the spinning disc. This is a first study providing quantitative microscopic data on dynamic spatio-temporal interactions of endosomes during root hair tip growth.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
—
Volume of the periodical
6
Issue of the periodical within the volume
JAN
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
"1262-1"-"1262-15"
UT code for WoS article
000368891500001
EID of the result in the Scopus database
2-s2.0-84956654095