Whole transcriptome analysis of transgenic barley with altered cytokinin homeostasis and increased tolerance to drought stress
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33162184" target="_blank" >RIV/61989592:15310/16:33162184 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/16:33162184
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S1871678416000133" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1871678416000133</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nbt.2016.01.010" target="_blank" >10.1016/j.nbt.2016.01.010</a>
Alternative languages
Result language
angličtina
Original language name
Whole transcriptome analysis of transgenic barley with altered cytokinin homeostasis and increased tolerance to drought stress
Original language description
Cytokinin plant hormones have been shown to play an important role in plant response to abiotic stresses. Herein, we expand upon our previous findings regarding preparation of novel transgenic barley lines overexpressing cytokinin dehydrogenase 1 gene from Arabidopsis under the control of mild root-specific promotor of maize β-glycosidase. These lines showed drought-tolerant phenotype mainly due to alteration of root architecture and stronger lignification of root tissue. A detailed transcriptomic analysis of roots of transgenic plants subjected to revitalization after drought stress revealed attenuated response through the HvHK3 cytokinin receptor and up-regulation of two transcription factors implicated in stress responses and abscisic acid sensitivity. Increased expression of several genes involved in the phenylpropanoid pathway as well as of genes encoding arogenate dehydratase/lyase participating in phenylalanine synthesis was found in roots during revitalization. Although more precursors of lignin synthesis were present in roots after drought stress, final lignin accumulation did not change compared to that in plants grown under optimal conditions. Changes in transcriptome indicated a higher auxin turnover in transgenic roots. The same analysis in leaves revealed that genes encoding putative enzymes responsible for production of jasmonates and other volatile compounds were up-regulated. Although transgenic barley leaves showed lower chlorophyll content and down-regulation of genes encoding proteins involved in photosynthesis than did wild-type plants when cultivated under optimal conditions, they did show a tendency to return to initial photochemical activities faster than did wild-type leaves when re-watered after severe drought stress.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Biotechnology
ISSN
1871-6784
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
5
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
16
Pages from-to
676-691
UT code for WoS article
000378026400010
EID of the result in the Scopus database
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