Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F17%3A73584034" target="_blank" >RIV/61989592:15310/17:73584034 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/jxb/article/68/20/5539/4560671" target="_blank" >https://academic.oup.com/jxb/article/68/20/5539/4560671</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/erx359" target="_blank" >10.1093/jxb/erx359</a>

Result language

angličtina

Original language name

Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal

Original language description

The heterotrophic lifestyle of parasitic plants relies on the development of the haustorium, a specific infectious organ required for attachment to host roots. While haustorium development is initiated upon chemodetection of host-derived molecules in hemiparasitic plants, the induction of haustorium formation remains largely unknown in holoparasitic species such as Phelipanche ramosa. This work demonstrates that the root exudates of the host plant Brassica napus contain allelochemicals displaying haustorium-inducing activity on P. ramosa germinating seeds, which increases the parasite aggressiveness. A de novo assembled transcriptome and microarray approach with P. ramosa during early haustorium formation upon treatment with B. napus root exudates allowed the identification of differentially expressed genes involved in hormone signaling. Bioassays using exogenous cytokinins and the specific cytokinin receptor inhibitor PI-55 showed that cytokinins induced haustorium formation and increased parasite aggressiveness. Root exudates triggered the expression of cytokinin-responsive genes during early haustorium development in germinated seeds, and bio-guided UPLC-ESI(+)-/MS/MS analysis showed that these exudates contain a cytokinin with dihydrozeatin characteristics. These results suggest that cytokinins constitutively exudated from host roots play a major role in haustorium formation and aggressiveness in P. ramosa.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

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Volume of the periodical

68

Issue of the periodical within the volume

20

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

5539-5552

UT code for WoS article

000417159100012

EID of the result in the Scopus database

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