High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F17%3A73585172" target="_blank" >RIV/61989592:15310/17:73585172 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/17:00476246 RIV/62156489:43210/17:43912530

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fpls.2017.00947/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fpls.2017.00947/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2017.00947" target="_blank" >10.3389/fpls.2017.00947</a>

Result language

angličtina

Original language name

High Throughput Screening Method for Identifying Potential Agonists and Antagonists of Arabidopsis thaliana Cytokinin Receptor CRE1/AHK4

Original language description

The CRE1/AHK4 cytokinin receptor is an important component of plants&apos; hormone signaling systems, and compounds that can alter its activity have potential utility for studying the receptor&apos;s functions and/or developing new plant growth regulators. A high throughput method was developed for screening compounds with agonist or antagonist properties toward the CRE1/AHK4 cytokinin receptor in a single experiment using the Nanodrop II liquid handling system and 384-well plates. Potential ligands are screened directly, using a reporter system in which receptor signaling activity triggers expression of beta-galactosidase in Escherichia coli. This enzyme generates a fluorescent product from a non-fluorescent substrate, allowing the agonistic/antagonistic behavior of tested compounds to be assayed in relation to that of an internal standard (here the natural ligand, trans-zeatin). The method includes a robust control procedure to determine false positive or false negative effects of the tested compounds arising from their fluorescent or fluorescent-quenching properties. The presented method enables robust, automated screening of large libraries of compounds for ability to activate or inhibit the Arabidopsis thaliana cytokinin receptor CRE1/AHK4.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

JUN

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

"974-1"-"947-11"

UT code for WoS article

000402858100002

EID of the result in the Scopus database

2-s2.0-85021055465