Complete genome sequence of a novel bromovirus infecting elderberry (Sambucus nigra L.) in the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73582203" target="_blank" >RIV/61989592:15310/18:73582203 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs00705-017-3629-1" target="_blank" >https://link.springer.com/article/10.1007%2Fs00705-017-3629-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00705-017-3629-1" target="_blank" >10.1007/s00705-017-3629-1</a>

Result language
angličtina
Original language name
Complete genome sequence of a novel bromovirus infecting elderberry (Sambucus nigra L.) in the Czech Republic
Original language description
The genus Bromovirus currently contains six species whose members have relatively narrow host ranges. In the present work, a new bromovirus infecting elderberry (Sambucus nigra L.) is reported. dsRNA was purified and sequenced by next-generation sequencing, and with minimal additional completion by Sanger sequencing, the full tripartite genome was obtained. RNA1 is 3241 nt long and contains ORF1 (1a protein), RNA2 is 2810 nt long and contains ORF2 (2a protein), and RNA3 is 2244 nt long and contains ORF3a (movement protein) and ORF3b (coat protein, CP), separated by an intercistronic poly(A) stretch. Proteins 1a and 2a showed highest sequence identity (69.9% and 69.4%) to the corresponding proteins of melandrium yellow fleck virus. The coat protein showed highest sequence identity (67.9%) to that of brome mosaic virus. The genome shows a typical bromovirus organisation comprising of all the conserved protein domains within the genus. Phylogenetic analysis supports the assignment of this virus as a new member of the genus Bromovirus, for which the name “sambucus virus S” (SVS) is proposed.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology

Project
<a href="/en/project/LD15048" target="_blank" >LD15048: Využití next generation sequencing ve studiu viromu ovocných dřevin</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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