Molecular mechanisms of photoadaptation of photosystem I supercomplex of in an evolutionary cyanobacterial/algal intermediate
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73582983" target="_blank" >RIV/61989592:15310/18:73582983 - isvavai.cz</a>
Result on the web
<a href="http://www.plantphysiol.org/content/176/2/1433" target="_blank" >http://www.plantphysiol.org/content/176/2/1433</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1104/pp.17.01022" target="_blank" >10.1104/pp.17.01022</a>
Alternative languages
Result language
angličtina
Original language name
Molecular mechanisms of photoadaptation of photosystem I supercomplex of in an evolutionary cyanobacterial/algal intermediate
Original language description
The monomeric PSI-LHCI supercomplex from an extremophilic red alga Cyanidioschyzon merolae represents an intermediate evolutionary link between the cyanobacterial PSI reaction centre and its green algal/higher plant counterpart. We show that the C. merolae PSI-LHCI supercomplex is characterized by robustness in various extreme conditions. By combination of biochemical, spectroscopic, mass spectrometry and electron microscopy/single particle analyses we dissected three molecular mechanisms underlying inherent robustness of C. merolae PSI-LHCI supercomplex: (i), the accumulation of a photoprotective zeaxanthin in the LHCI antenna and the PSI reaction centre; (ii), structural remodelling of the LHCI antenna and adjustment of its effective absorption cross-section; and (iii), dynamic re-adjustment of the stoichiometry of the two PSI-LHCI isomers and changes in the oligomeric state of the PSI-LHCI supercomplex, accompanied by dissociation of the PsaK core subunit. We show that the largest low light-treated C. merolae PSI-LHCI supercomplex can bind up to 8 Lhcr antenna subunits which are organized as 2 rows on the PsaF/PsaJ side of the core complex. Under our experimental conditions, we found no evidence of functional coupling of the phycobilisomes with the PSI-LHCI supercomplex purified from various light conditions, suggesting that the putative association of this antenna with PSI supercomplex is absent or transient, or may be lost during the purification procedure.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Physiology
ISSN
0032-0889
e-ISSN
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Volume of the periodical
176
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
19
Pages from-to
1433-1451
UT code for WoS article
000424285500037
EID of the result in the Scopus database
2-s2.0-85041730163