Regulation of enzyme activities in carnivorous pitcher plants of the genus Nepenthes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73588606" target="_blank" >RIV/61989592:15310/18:73588606 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs00425-018-2917-7" target="_blank" >https://link.springer.com/article/10.1007%2Fs00425-018-2917-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00425-018-2917-7" target="_blank" >10.1007/s00425-018-2917-7</a>

Result language

angličtina

Original language name

Regulation of enzyme activities in carnivorous pitcher plants of the genus Nepenthes

Original language description

Carnivorous plants of the genus Nepenthes have evolved passive pitcher traps for prey capture. In this study, we investigated the ability of chemical signals from a prey (chitin, protein, and ammonium) to induce transcription and synthesis of digestive enzymes in Nepenthes × Mixta. We used real-time PCR and specific antibodies generated against the aspartic proteases nepenthesins, and type III and type IV chitinases to investigate the induction of digestive enzyme synthesis in response to different chemical stimuli from the prey. Transcription of nepenthesins was strongly induced by ammonium, protein and live prey; chitin induced transcription only very slightly. This is in accordance with the amount of released enzyme and proteolytic activity in the digestive fluid. Although transcription of type III chitinase was induced by all investigated stimuli, a significant accumulation of the enzyme in the digestive fluid was found mainly after protein and live prey addition. Protein and live prey were also the best inducers for accumulation of type IV chitinase in the digestive fluid. Although ammonium strongly induced transcription of all investigated genes probably through membrane depolarization, strong acidification of the digestive fluid affected stability and abundance of both chitinases in the digestive fluid. The study showed that the proteins are universal inductors of enzyme activities in carnivorous pitcher plants best mimicking the presence of insect prey. This is not surprising, because proteins are a much valuable source of nitrogen, superior to chitin. Extensive vesicular activity was observed in prey-activated glands.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10610 - Biophysics

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLANTA

ISSN

0032-0935

e-ISSN

—

Volume of the periodical

248

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

14

Pages from-to

"451–464"

UT code for WoS article

000437738900013

EID of the result in the Scopus database

2-s2.0-85047166884