Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73589790" target="_blank" >RIV/61989592:15310/18:73589790 - isvavai.cz</a>

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fpls.2018.00371/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fpls.2018.00371/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2018.00371" target="_blank" >10.3389/fpls.2018.00371</a>

Result language

angličtina

Original language name

Gene Expression Pattern and Protein Localization of Arabidopsis Phospholipase D Alpha 1 Revealed by Advanced Light-Sheet and Super-Resolution Microscopy

Original language description

Phospholipase D alpha 1 (PLD alpha 1, At3g15730) and its product phosphatidic acid (PA) are involved in a variety of cellular and physiological processes, such as cytoskeletal remodeling, regulation of stomatal closure and opening, as well as biotic and abiotic stress signaling. Here we aimed to study developmental expression patterns and subcellular localization of PLD alpha 1 in Arabidopsis using advanced microscopy methods such as light-sheet fluorescencemicroscopy (LSFM) and structured illumination microscopy (SIM). We complemented two knockout pld alpha 1 mutants with a YFP-tagged PLD alpha 1 expressed under the PLD alpha 1 native promoter in order to study developmental expression pattern and subcellular localization of PLD alpha 1 in Arabidopsis thaliana under natural conditions. Imaging of tissue-specific and developmentally-regulated localization of YFP-tagged PLD alpha 1 by LSFM in roots of growing seedlings showed accumulation of PLD alpha 1-YFP in the root cap and the rhizodermis. Expression of PLD alpha 1-YFP in the rhizodermis was considerably higher in trichoblasts before and during root hair formation and growth. Thus, PLD alpha 1-YFP accumulated in emerging root hairs and in the tips of growing root hairs. PLD alpha 1-YFP showed cytoplasmic subcellular localization in root cap cells and in cells of the root transition zone. In aerial parts of plants PLD alpha 1-YFP was also localized in the cytoplasm showing enhanced accumulation in the cortical cytoplasmic layer of epidermal non-dividing cells of hypocotyls, leaves, and leaf petioles. However, in dividing cells of root apical meristem and leaf petiole epidermis PLD alpha 1-YFP was enriched in mitotic spindles and phragmoplasts, as revealed by co-visualization with microtubules. Finally, super-resolution SIM imaging revealed association of PLD alpha 1-YFP with both microtubules and clathrin-coated vesicles (CCVs) and pits (CCPs). In conclusion, this study shows the developmentally-controlled expression and subcellular localization of PLD alpha 1 in dividing and non-dividing Arabidopsis cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10605 - Developmental biology

Project

<a href="/en/project/GA16-22044S" target="_blank" >GA16-22044S: Regulation of phospholipase D alpha 1 function by MPK3-dependent phosphorylation</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

MAR

Country of publishing house

CH - SWITZERLAND

Number of pages

21

Pages from-to

"371-1"-"371-21"

UT code for WoS article

000427928800001

EID of the result in the Scopus database

2-s2.0-85045444953